LOCUS       40924_37088       12502 bp DNA     circular SYN 18-DEC-2018
DEFINITION  RNAi cloning vector pRISE DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Kondo T, Inagaki S, Yasuda K, Kageyama Y.
  TITLE     Rapid construction of Drosophila RNAi transgenes using pRISE, a 
            P-element-mediated transformation vector exploiting an in vitro 
            recombination system
  JOURNAL   Genes Genet. Syst. 81 (2), 129-134 (2006)
  PUBMED    16755136
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   Kondo T, Inagaki S, Kageyama Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2006) Takefumi Kondo, Nara Inst. Sci. Tech., Grad.
            Sch. Biol. Sci.; 8916-5 Takayama, Ikoma, Nara 630-0192, Japan 
            (E-mail:ta-kondo@bs.naist.jp, Tel:81-743-72-5554, 
            Fax:81-743-72-5559)
REFERENCE   3  (bases 1 to 12502)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Genet. Syst."; date: "2006"; volume: "81"; issue: "2"; pages: 
            "129-134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUN-2006) Takefumi Kondo, Nara Inst. Sci. Tech., Grad. Sch. 
            Biol. Sci."; volume: " 8916-5 Takayama, Ikoma, Nara 630-0192, Japan 
            (E-mail:ta-kondo@bs.naist.jp, Tel:81-743-72-5554, Fax"; pages: 
            "81-743-72-5559"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..586
                     /label=P element 5' end
     misc_feature    complement(602..4728)
                     /label=mini-white
                     /note="mini-white"
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     polyA_signal    complement(4756..4890)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5161..5181)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(5311..5376)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    5460..5584
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5609..5639
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5693..6349
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6694..6996
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(7040..7164)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    complement(7175..7321)
                     /label=fushi-tarazu intron
                     /note="fushi-tarazu intron"
     protein_bind    7344..7468
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7512..7814)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(8159..8815)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(8869..8899)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(8924..9048)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(9098..9336)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    complement(9355..9449)
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    9510..9742
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        10544..10648
                     /label=AmpR promoter
     CDS             10649..11506
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11680..12268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtact tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatatccag tgcagtaaaa aataaaaaaa aaaaatgttt
     4681 ttttttatct actttccgca aaaatgggtt ttattaactt acatacatac tagaattagg
     4741 ccttctagtg gatccgatcc agacatgata agatacattg atgagtttgg acaaaccaca
     4801 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4861 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4921 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4981 atggctgatt atgatctcta gtcaaggcac tatacatcaa atattttcca taattttctt
     5041 gtatagcagt gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt
     5101 actaaacaca gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc
     5161 tacctttctc ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc
     5221 actagatggc atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg
     5281 ctcccattca tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag
     5341 tttaacacat tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg
     5401 tagtttgtcc aattatgtca caccacagaa gtaaggttcc ttcacaaaga tcctctagaa
     5461 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     5521 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     5581 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     5641 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     5701 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     5761 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     5821 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     5881 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     5941 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     6001 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     6061 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     6121 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     6181 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     6241 gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag
     6301 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
     6361 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     6421 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag
     6481 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     6541 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     6601 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     6661 gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa
     6721 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     6781 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     6841 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     6901 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     6961 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     7021 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     7081 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     7141 ttcagctttc ttgtacaaag tggtcacagt cgacctgtaa gcataagcaa agaaaaaatg
     7201 ggttagctaa tgagttttat aactgcgtat tgtttgaaaa tatcaaaaat gtatacaaaa
     7261 acatattttc aatattttca aagtgtattt ttaggggttg ttaatcgtgt gtgatgccta
     7321 ccttctagag gtaccctcga atcaccactt tgtacaagaa agctgaacga gaaacgtaaa
     7381 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     7441 gtaaaacaca acatatccag tcactatggt cgacctgcag actggctgtg tataagggag
     7501 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
     7561 tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg
     7621 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact
     7681 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca
     7741 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
     7801 accttaaact gcatttcacc agtccctgtt ctcgtcagca aaagagccgt tcatttcaat
     7861 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag
     7921 acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga catcatatat
     7981 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca
     8041 cacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat
     8101 cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca cgcgtttacg
     8161 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     8221 agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     8281 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     8341 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     8401 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     8461 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     8521 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     8581 ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     8641 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     8701 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     8761 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     8821 cttccttagc tcctgaaaat ctcgccggat cctaactcaa aatccacaca ttatacgagc
     8881 cggaagcata aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg
     8941 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     9001 atttatatca ttttacgttt ctcgttcagc ttttttgtac aaacttgtga ttcgagccgc
     9061 ggccgcagat ctgttaacga attcccaatt ccctattcag agttctcttc ttgtattcaa
     9121 taattacttc ttggcagatt tcagtagttg cagttgattt acttggttgc tggttacttt
     9181 taattgattc actttaactt gcactttact gcagattgtt tagcttgttc agctgcgctt
     9241 gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga attgaattgt
     9301 cgctccgtag acgaagcgcc tctatttata ctccggcgct cgctagagtc tccgctcgga
     9361 ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga
     9421 cagtactccg ctcggaggac agtactccga cctgcaggca tgcaagcttg gatccggaga
     9481 gctcccaacc gttggatgca gccaagcttt gcgtactcgc aaattattaa aaataaaact
     9541 ttaaaaataa tttcgtctaa ttaatattat gagttaattc aaaccccacg gacatgctaa
     9601 gggttaatca acaatcatat cgctgtctca ctcagactca atacgacact cagaatacta
     9661 ttcctttcac tcgcacttat tgcaagcata cgttaagtgg atgtctcttg ccgacgggac
     9721 caccttatgt tatttcatca tggtctggcc attctcatcg tgagcttccg ggtgctcgca
     9781 tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
     9841 tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa
     9901 agctaatgta attatatttg tgccaataaa aacaagatat gacctataga atacaagtat
     9961 ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac
    10021 acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt
    10081 ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac
    10141 gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt
    10201 tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg
    10261 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
    10321 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
    10381 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
    10441 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
    10501 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    10561 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    10621 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    10681 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    10741 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    10801 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    10861 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
    10921 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10981 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    11041 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    11101 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    11161 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    11221 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    11281 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    11341 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    11401 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    11461 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    11521 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    11581 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    11641 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    11701 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    11761 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    11821 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    11881 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    11941 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    12001 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    12061 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    12121 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    12181 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    12241 ctcgtcaggg gggcggagcc tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc
    12301 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
    12361 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
    12421 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
    12481 gctggactac gtgggtctgg cc
//
